Compute key CZE performance parameters — capillary volume, electric field strength, electrophoretic and electroosmotic mobility, theoretical plates (N), and resolution (Rs) — from capillary dimensions, voltage, and migration times. Use this tool when developing or optimizing a CZE method: resolution of 1.5 or higher indicates baseline separation, while plate counts exceeding 200,000 per meter are typical for well-performing systems. The capillary geometry section helps calculate volumes for injection and buffer consumption estimates.

About CZE Parameters

In capillary zone electrophoresis, separation performance depends on the interplay of capillary geometry, applied voltage, buffer properties, and analyte mobilities. The number of theoretical plates (N) follows N = 16(tₘ/W)², while resolution between two peaks is given by Rs = 2(tₘ₂ − tₘ₁)/(W₁ + W₂). Electrophoretic mobility (µₑₚ) characterizes an analyte’s intrinsic migration velocity per unit field strength, and the electroosmotic flow (EOF) mobility (µₑₒₓ) reflects the bulk flow driven by the charged capillary wall. Capillary inner diameter directly affects both separation efficiency (through Joule heating) and detection sensitivity (through optical path length), with 25–75 µm ID capillaries being typical for most applications.