Calculate the melting temperature (Tm) of your oligonucleotide sequence using this simple calculator.

About Tm Calculation

The melting temperature (Tm) is the temperature at which half of the DNA duplexes dissociate into single strands. This calculator uses a simplified method (a variation of the Wallace rule) for Tm estimation. More sophisticated algorithms that consider factors like salt concentration and nearest-neighbor interactions exist, but this provides a reasonable approximation for many purposes. For precise Tm calculations, especially for critical applications, it’s recommended to use dedicated software or consult specialized resources.

Important Considerations:

• Simplified Calculation: The formula used here is a simplification and may not be accurate for all sequences, especially those with complex structures or modified bases.
• Salt Concentration: The salt concentration significantly affects Tm. Make sure to input the correct value.
• More Accurate Methods: For more precise Tm predictions, consider using specialized software or online tools that implement more advanced algorithms. These tools often take into account nearest-neighbor interactions, specific salt concentrations, and other factors.