Scan all six reading frames of a nucleotide sequence to identify open reading frames bounded by start (ATG) and stop codons, with adjustable minimum length filtering. Use this tool when annotating a newly assembled genome, validating a cloned gene, or searching for potential protein-coding regions in uncharacterized sequence. The graphical map displays ORF positions across all frames, and longer ORFs are statistically more likely to represent genuine protein-coding genes — short ORFs below 100 codons are often false positives from random sequence.

How It Works

• Scans all 6 reading frames (3 forward, 3 reverse complement)
• Identifies ORFs from start codon (ATG) to stop codon (TAA/TAG/TGA)
• Adjustable minimum ORF length filter
• Each ORF is translated to its protein sequence
• Graphical map provides a visual overview of ORF positions