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Restriction Mapper

May 21, 2026 · Updated: May 21, 2026

Map restriction enzyme recognition sites on a DNA sequence using a library of over 150 enzymes, with automatic fragment size calculation and a visual linear map of cut positions. Use this tool when planning a subcloning strategy — it shows exactly where each enzyme cuts, the resulting fragment lengths, and helps you identify which enzymes will release your insert without cutting it internally. The sortable fragment table and downloadable CSV make it easy to communicate your cloning plan or document expected gel band patterns.

Restriction Mapper

Features

  • Enzyme Library: 150+ restriction enzymes with recognition sites and cut positions
  • Fragment Analysis: Automatic fragment size calculation with sortable table
  • Visual SVG Map: Linear map with tick marks at each cut site
  • CSV Export: Download fragment table as CSV
  • Search & Filter: Quickly find enzymes by name or recognition sequence