Compute fundamental sequence statistics including GC content, GC skew, molecular weight, base composition, and extinction coefficient for any DNA or RNA sequence. Use this tool when characterizing a newly sequenced gene, designing qPCR assays where GC content affects annealing temperature, or reporting sequence properties in a publication. GC content and skew values also provide clues about GC-rich regions, origins of replication, and potential horizontal gene transfer events that may be relevant to your analysis.

Features

• GC Content & Skew: GC percentage and (G-C)/(G+C) skew
• Base Composition: Individual nucleotide counts and donut chart
• Molecular Weight: Estimated MW for ssDNA and dsDNA
• Extinction Coefficient: Approximate molar extinction coefficient
• FASTA Support: Paste sequences with or without FASTA headers