Solve for any variable in the Beer-Lambert equation A = εcl — absorbance, concentration, molar absorptivity, or path length — by entering the other three values. Use this tool when quantifying DNA, RNA, or protein concentration by UV-Vis spectrophotometry, or when determining the molar absorptivity of a newly synthesized compound. Reliable measurements depend on staying within the linear absorbance range (typically A below 1.5), and the tool helps verify that your sample dilution is appropriate for accurate quantitation.

About the Beer-Lambert Law

The Beer-Lambert Law (A = εcl) describes the linear relationship between absorbance (A) and concentration (c), path length (l), and molar absorptivity (ε) of a sample. It is fundamental to UV-Vis spectroscopy and spectrophotometric analysis.