计算机模拟双酶切工具 使用包含超过150种酶的庞大酶库,对DNA序列进行虚拟限制性内切酶消化。该工具可精确计算切割位置、片段大小,并提供每个片段的完整序列——非常适合用于规划克隆策略、验证 PCR 产物或预测电泳图谱。 碱性磷酸酯酶库 150+ 酶 DNA序列输入 酶 1 -- 无选择 -- AanI (PsiI)AarIAasI (DrdI)AatIIAcc65I (Asp718I)AdeI (DraIII)AjiI (BmgBI)AjuIAlfIAloIAluIAlw21I (BsiHKAI)Alw26I (BsmAI)Alw44I (ApaLI)ApaIBamHIBauI (BssSI)BclIBcnI (NciI)BcuI (SpeI)BdaIBfiI (BmrI)BfmI (SfcI)BfuI (BciVI)BglIBglIIBme1390I (ScrFI)BoxI (PshAI)BpiI (BbsI)BplIBpu10IBpu1102I (BlpI)BseDI (BsaJI)BseGI (BtsCI)BseJI (BsaBI)BseLI (BslI)BseMI (BsrDI)BseMII (BspCNI)BseNI (BsrI)BseSI (Bme1580I)BseXI (BbvI)Bsh1236I (BstUI)Bsh1285I (BsiEI)BshNI (BanI)BshTI (AgeI)Bsp119I (BstBI)Bsp120I (PspOMI)Bsp1407I (BsrGI)Bsp143I (Sau3AI)Bsp68I (NruI)BspLI (NlaIV)BspOI (BmtI)BspPI (AlwI)BspTI (AflII)Bst1107I (BstZ17I)BstXIBsu15I (ClaI)BsuRI (HaeIII)BveI (BspMI)CaiI (AlwNI)Cfr10I (BsrFI)Cfr13I (Sau96I)Cfr42I (SacII)Cfr9I (XmaI)CfrI (EaeI)CpoI (RsrII)CseI (HgaI)Csp6I (CviQI)DpnIDraIEam1104I (EarI)Eam1105I (AhdI)Ecl136II (EcoICRI)Eco105I (SnaBI)Eco130I (StyI)Eco147I (StuI)Eco24I (BanII)Eco31I (BsaI)Eco32I (EcoRV)Eco47I (AvaII)Eco47III (AfeI)Eco52I (EagI)Eco57I (AcuI)Eco57MIEco72I (PmlI)Eco81I (Bsu36I)Eco88I (AvaI)Eco91I (BstEII)EcoO109I (DraII)EcoRIEcoRIIEheI (SfoI)Esp3I (BsmBI)FaqI (BsmFI)FspAIFspBI (BfaI)GsuI (BpmI)HhaIHin1I (BsaHI)Hin1II (NlaIII)Hin4IHin6I (HinP1I)HincII (HindII)HindIIIHinfIHpaIIHphIHpy8I (MjaIV)HpyF10VI (MwoI)HpyF3I (DdeI)Kpn2I (BspEI)KpnIKspAI (HpaI)LguI (SapI)Lsp1109I (BbvI)LweI (SfaNI)MauBIMbiI (BsrBI)MboIMboIIMlsI (MscI)MluIMnlIMph1103I (NsiI)MreI (Sse232I)MspI (HpaII)MssI (PmeI)MunI (MfeI)Mva1269I (BsmI)MvaI (BstNI)NcoINdeINheINmuCI (Tsp45I)NotINsbI (FspI)OliI (AleI)PacIPaeI (SphI)PagI (BspHI)PasIPauI (BssHII)PdiI (NaeI)PdmI (XmnI)PfeI (TfiI)Pfl23II (BsiWI)PfoIPpiIPpu21I (BsaAI)PscI (PciI)Psp1406I (AclI)Psp5II (PpuMI)PstIPsuI (BstYI)PsyI (Tth111I)PvuIPvuIIRsaIRseI (MslI)SacISalISatI (Fnu4HI)ScaISchI (MlyI)SdaI (SbfI)SduI (Bsp1286I)SfaAI (AsiSI)SfiISgrDISgsI (AscI)SmaISmiI (SwaI)SmoI (SmlI)SmuI (FauI)SsiI (AciI)SspDI (KasI)SspITaaI (HpyCH4III)TaiI (MaeII)TaqITasI (Tsp509I)TatITauITru1I (MseI)TscAI (TspRI)TsoITstIVan91I (PflMI)VspI (AseI)XagI (EcoNI)XapI (ApoI)XbaIXceI (NspI)XhoIXmaJI (AvrII)XmiI (AccI) 酶 2 -- 无选择 -- AanI (PsiI)AarIAasI (DrdI)AatIIAcc65I (Asp718I)AdeI (DraIII)AjiI (BmgBI)AjuIAlfIAloIAluIAlw21I (BsiHKAI)Alw26I (BsmAI)Alw44I (ApaLI)ApaIBamHIBauI (BssSI)BclIBcnI (NciI)BcuI (SpeI)BdaIBfiI (BmrI)BfmI (SfcI)BfuI (BciVI)BglIBglIIBme1390I (ScrFI)BoxI (PshAI)BpiI (BbsI)BplIBpu10IBpu1102I (BlpI)BseDI (BsaJI)BseGI (BtsCI)BseJI (BsaBI)BseLI (BslI)BseMI (BsrDI)BseMII (BspCNI)BseNI (BsrI)BseSI (Bme1580I)BseXI (BbvI)Bsh1236I (BstUI)Bsh1285I (BsiEI)BshNI (BanI)BshTI (AgeI)Bsp119I (BstBI)Bsp120I (PspOMI)Bsp1407I (BsrGI)Bsp143I (Sau3AI)Bsp68I (NruI)BspLI (NlaIV)BspOI (BmtI)BspPI (AlwI)BspTI (AflII)Bst1107I (BstZ17I)BstXIBsu15I (ClaI)BsuRI (HaeIII)BveI (BspMI)CaiI (AlwNI)Cfr10I (BsrFI)Cfr13I (Sau96I)Cfr42I (SacII)Cfr9I (XmaI)CfrI (EaeI)CpoI (RsrII)CseI (HgaI)Csp6I (CviQI)DpnIDraIEam1104I (EarI)Eam1105I (AhdI)Ecl136II (EcoICRI)Eco105I (SnaBI)Eco130I (StyI)Eco147I (StuI)Eco24I (BanII)Eco31I (BsaI)Eco32I (EcoRV)Eco47I (AvaII)Eco47III (AfeI)Eco52I (EagI)Eco57I (AcuI)Eco57MIEco72I (PmlI)Eco81I (Bsu36I)Eco88I (AvaI)Eco91I (BstEII)EcoO109I (DraII)EcoRIEcoRIIEheI (SfoI)Esp3I (BsmBI)FaqI (BsmFI)FspAIFspBI (BfaI)GsuI (BpmI)HhaIHin1I (BsaHI)Hin1II (NlaIII)Hin4IHin6I (HinP1I)HincII (HindII)HindIIIHinfIHpaIIHphIHpy8I (MjaIV)HpyF10VI (MwoI)HpyF3I (DdeI)Kpn2I (BspEI)KpnIKspAI (HpaI)LguI (SapI)Lsp1109I (BbvI)LweI (SfaNI)MauBIMbiI (BsrBI)MboIMboIIMlsI (MscI)MluIMnlIMph1103I (NsiI)MreI (Sse232I)MspI (HpaII)MssI (PmeI)MunI (MfeI)Mva1269I (BsmI)MvaI (BstNI)NcoINdeINheINmuCI (Tsp45I)NotINsbI (FspI)OliI (AleI)PacIPaeI (SphI)PagI (BspHI)PasIPauI (BssHII)PdiI (NaeI)PdmI (XmnI)PfeI (TfiI)Pfl23II (BsiWI)PfoIPpiIPpu21I (BsaAI)PscI (PciI)Psp1406I (AclI)Psp5II (PpuMI)PstIPsuI (BstYI)PsyI (Tth111I)PvuIPvuIIRsaIRseI (MslI)SacISalISatI (Fnu4HI)ScaISchI (MlyI)SdaI (SbfI)SduI (Bsp1286I)SfaAI (AsiSI)SfiISgrDISgsI (AscI)SmaISmiI (SwaI)SmoI (SmlI)SmuI (FauI)SsiI (AciI)SspDI (KasI)SspITaaI (HpyCH4III)TaiI (MaeII)TaqITasI (Tsp509I)TatITauITru1I (MseI)TscAI (TspRI)TsoITstIVan91I (PflMI)VspI (AseI)XagI (EcoNI)XapI (ApoI)XbaIXceI (NspI)XhoIXmaJI (AvrII)XmiI (AccI) 酶 3 -- 无选择 -- AanI (PsiI)AarIAasI (DrdI)AatIIAcc65I (Asp718I)AdeI (DraIII)AjiI (BmgBI)AjuIAlfIAloIAluIAlw21I (BsiHKAI)Alw26I (BsmAI)Alw44I (ApaLI)ApaIBamHIBauI (BssSI)BclIBcnI (NciI)BcuI (SpeI)BdaIBfiI (BmrI)BfmI (SfcI)BfuI (BciVI)BglIBglIIBme1390I (ScrFI)BoxI (PshAI)BpiI (BbsI)BplIBpu10IBpu1102I (BlpI)BseDI (BsaJI)BseGI (BtsCI)BseJI (BsaBI)BseLI (BslI)BseMI (BsrDI)BseMII (BspCNI)BseNI (BsrI)BseSI (Bme1580I)BseXI (BbvI)Bsh1236I (BstUI)Bsh1285I (BsiEI)BshNI (BanI)BshTI (AgeI)Bsp119I (BstBI)Bsp120I (PspOMI)Bsp1407I (BsrGI)Bsp143I (Sau3AI)Bsp68I (NruI)BspLI (NlaIV)BspOI (BmtI)BspPI (AlwI)BspTI (AflII)Bst1107I (BstZ17I)BstXIBsu15I (ClaI)BsuRI (HaeIII)BveI (BspMI)CaiI (AlwNI)Cfr10I (BsrFI)Cfr13I (Sau96I)Cfr42I (SacII)Cfr9I (XmaI)CfrI (EaeI)CpoI (RsrII)CseI (HgaI)Csp6I (CviQI)DpnIDraIEam1104I (EarI)Eam1105I (AhdI)Ecl136II (EcoICRI)Eco105I (SnaBI)Eco130I (StyI)Eco147I (StuI)Eco24I (BanII)Eco31I (BsaI)Eco32I (EcoRV)Eco47I (AvaII)Eco47III (AfeI)Eco52I (EagI)Eco57I (AcuI)Eco57MIEco72I (PmlI)Eco81I (Bsu36I)Eco88I (AvaI)Eco91I (BstEII)EcoO109I (DraII)EcoRIEcoRIIEheI (SfoI)Esp3I (BsmBI)FaqI (BsmFI)FspAIFspBI (BfaI)GsuI (BpmI)HhaIHin1I (BsaHI)Hin1II (NlaIII)Hin4IHin6I (HinP1I)HincII (HindII)HindIIIHinfIHpaIIHphIHpy8I (MjaIV)HpyF10VI (MwoI)HpyF3I (DdeI)Kpn2I (BspEI)KpnIKspAI (HpaI)LguI (SapI)Lsp1109I (BbvI)LweI (SfaNI)MauBIMbiI (BsrBI)MboIMboIIMlsI (MscI)MluIMnlIMph1103I (NsiI)MreI (Sse232I)MspI (HpaII)MssI (PmeI)MunI (MfeI)Mva1269I (BsmI)MvaI (BstNI)NcoINdeINheINmuCI (Tsp45I)NotINsbI (FspI)OliI (AleI)PacIPaeI (SphI)PagI (BspHI)PasIPauI (BssHII)PdiI (NaeI)PdmI (XmnI)PfeI (TfiI)Pfl23II (BsiWI)PfoIPpiIPpu21I (BsaAI)PscI (PciI)Psp1406I (AclI)Psp5II (PpuMI)PstIPsuI (BstYI)PsyI (Tth111I)PvuIPvuIIRsaIRseI (MslI)SacISalISatI (Fnu4HI)ScaISchI (MlyI)SdaI (SbfI)SduI (Bsp1286I)SfaAI (AsiSI)SfiISgrDISgsI (AscI)SmaISmiI (SwaI)SmoI (SmlI)SmuI (FauI)SsiI (AciI)SspDI (KasI)SspITaaI (HpyCH4III)TaiI (MaeII)TaqITasI (Tsp509I)TatITauITru1I (MseI)TscAI (TspRI)TsoITstIVan91I (PflMI)VspI (AseI)XagI (EcoNI)XapI (ApoI)XbaIXceI (NspI)XhoIXmaJI (AvrII)XmiI (AccI) 运行模拟碎裂 No cut sites found for the selected enzyme(s) in this sequence. # 长度 端 (L/R) 操作