计算蛋白质序列的关键理化特性，包括分子量、理论 pI、给定 pH 下的净电荷、GRAVY 亲水评分、消光系数和不稳定指数。 在表征重组蛋白时使用此工具 - pI 指导离子交换色谱的缓冲液选择，GRAVY 分数预测溶解度和膜结合。 The instability index helps assess whether the protein is likely to remain stable in solution during purification and downstream applications.

计算属性

• Molecular Weight: Sum of amino acid residue masses + water
• Theoretical pI: Isoelectric point from ionizable group pKa values
• Charge at pH: Net charge using Henderson-Hasselbalch equation
• GRAVY Score: Grand average of hydropathy (Kyte-Doolittle)
• Extinction Coefficient: Molar absorptivity at 280 nm
• Instability Index: Predicts protein stability in vitro