Antigen Retrieval and IHC Methods
Antigen retrieval restores antibody accessibility in formalin-fixed tissue through heat-induced or enzymatic methods, determining IHC success.
IHC in Tumor Diagnosis and Classification
Immunohistochemistry is essential for classifying tumors of unknown origin, confirming lineage, and identifying specific tumor subtypes.
IHC Troubleshooting, Controls and Optimization
Systematic troubleshooting of immunohistochemistry identifies causes of weak staining, background, and false results through proper control evaluation.
Predictive and Prognostic IHC Markers
Predictive IHC markers guide therapy selection by identifying target expression, while prognostic markers estimate patient outcome independent of treatment.
Principles of Immunohistochemistry
Immunohistochemistry uses antibody-antigen binding to localize specific proteins in tissue sections, enabling cell typing, tumor classification, and biomarker detection.
