FPLC
Fast protein liquid chromatography is a moderate-pressure chromatography system designed for preparative purification of proteins and other biomolecules.
HIC
Hydrophobic interaction chromatography separates proteins based on surface hydrophobicity using a high-salt mobile phase and a mildly hydrophobic stationary phase.
RPC
Reversed-phase chromatography separates molecules based on hydrophobicity using a nonpolar stationary phase and a polar mobile phase.
Affinity Chromatography
Affinity chromatography exploits specific biological interactions between a ligand and target molecule for high-purity separations.
Hyphenated Techniques (GC-MS, LC-MS)
Hyphenated techniques couple separation methods with mass spectrometry to provide both separation and structural identification.
Introduction to Chromatography
Chromatography separates mixtures by distributing components between a stationary and mobile phase.
Method Development in Chromatography
Method development systematically optimizes chromatographic parameters to achieve adequate separation and analysis time.
Size-Exclusion Chromatography
Size-exclusion chromatography separates molecules by their hydrodynamic volume or molecular size.
Supercritical Fluid Chromatography
Supercritical fluid chromatography uses supercritical CO₂ as the mobile phase, combining advantages of GC and HPLC.
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