ATAC-Seq
Assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-Seq) maps genome-wide chromatin accessibility using a hyperactive Tn5 transposase.
Bisulfite Sequencing and DNA Methylation Analysis
Bisulfite sequencing detects 5-methylcytosine at single-base resolution by chemically converting unmethylated cytosines to uracil while leaving methylated cytosines unchanged.
TALENs and Zinc Finger Nucleases
TALENs and zinc finger nucleases (ZFNs) are programmable DNA-binding proteins that create targeted double-strand breaks for genome editing in eukaryotic cells.
Bacterial Transformation
Bacterial transformation introduces exogenous plasmid DNA into competent bacterial cells, enabling cloning, protein expression, and library construction.
CRISPR Screening
CRISPR screening uses pooled single-guide RNA libraries to identify genes essential for a phenotype of interest in a high-throughput, genome-wide manner.
Gateway and TOPO Cloning
Gateway recombinational cloning and TOPO cloning are rapid, PCR-based methods for [transferring DNA sequences between plasmids](/guides/recombinant-dna-technology.html) without restriction enzymes or ligase.
Gibson Assembly and Golden Gate Cloning
Gibson Assembly and Golden Gate cloning enable seamless, scarless assembly of multiple DNA fragments in a single reaction for synthetic biology and large construct engineering.
Transfection Methods
Transfection delivers nucleic acids into eukaryotic cells for studying gene function, protein expression, and genetic manipulation.
Cryopreservation and Cell Banking
Cryopreservation enables long-term storage of viable cells, tissues, and microorganisms by cooling them to sub-zero temperatures while preventing ice crystal damage.
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