Epigenomics: ATAC-Seq and Bisulfite Sequencing
ATAC-seq maps open chromatin regions to identify regulatory elements, while bisulfite sequencing profiles DNA methylation patterns across the genome.
Reporter Gene Assays
Reporter gene assays use easily measurable proteins to monitor gene expression, promoter activity, and cellular signaling pathways.
RNA Interference (siRNA, shRNA, miRNA)
RNA interference is a conserved cellular mechanism for sequence-specific gene silencing, standard to study gene function and identify therapeutic targets.
Targeted Genome Engineering: CRISPR Screening, TALENs, and Zinc Finger Nucleases
Targeted genome engineering encompasses a suite of tools, CRISPR screening, TALENs, and zinc finger nucleases, for precisely modifying or interrogating genes.
DNA and RNA Quantification Methods
Accurate quantification of nucleic acids is essential for downstream applications including sequencing, PCR, cloning, and microarray analysis.
Gel Extraction and PCR Purification
Gel extraction and PCR purification are the two standard methods for purifying DNA fragments after amplification or restriction digestion.
LAMP and Isothermal Amplification
Loop-mediated isothermal amplification and related methods amplify DNA at a constant temperature, enabling rapid, point-of-care [nucleic acid detection](/guides/dna-isolation.html).
Modern Cloning Methods: Gateway, Gibson Assembly, and Golden Gate
Modern cloning methods such as Gateway recombinational cloning, Gibson Assembly, and Golden Gate cloning enable seamless, modular DNA assembly without restriction enzyme digestion and ligation.
Transformation and Transfection Methods
Transformation and transfection are methods for introducing exogenous nucleic acids into bacterial and eukaryotic cells for genetic manipulation.