Fluorescence In Situ Hybridization
Fluorescence in situ hybridization uses fluorescently labeled DNA probes to detect and localize specific DNA sequences on chromosomes and in interphase nuclei.
In Situ Hybridization
In situ hybridization uses labeled complementary DNA or RNA probes to detect and localize specific nucleic acid sequences within intact cells, tissues, or chromosomes.
Phage Display
Phage display is a molecular biology technique that displays foreign peptides or proteins on the surface of bacteriophage particles, enabling selection and evolution of molecules with desired binding properties.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation is a method for mapping protein-DNA interactions in the genome, identifying where transcription factors and histone modifications are located.
CRISPR Applications Beyond Cas9
CRISPR technology has expanded far beyond genome editing with Cas9 to include base editing, prime editing, gene regulation, and diagnostic applications.
DNA Microarrays and Gene Expression Analysis
DNA microarrays allow simultaneous measurement of the expression levels of thousands of genes, providing a genome-wide view of cellular transcriptional activity.
EMSA and DNA Footprinting
EMSA and DNA footprinting are classic methods for studying protein-DNA interactions, revealing binding affinity, specificity, and the precise nucleotides contacted by proteins.
Flow Cytometry
Flow cytometry is a laser-based technology that rapidly measures physical and chemical properties of individual cells or particles in a fluid stream.
Yeast Two-Hybrid System
The yeast two-hybrid system detects protein-protein interactions in living yeast cells by reconstituting a functional transcription factor.
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