PCR Applications and Variants
The polymerase chain reaction has spawned numerous specialized variants and applications that extend its utility beyond simple DNA amplification.
Colony PCR
Colony PCR is a technique used to screen bacterial colonies for the presence of a correct DNA insert after cloning.
Digital PCR
Digital PCR (dPCR) is a technique that provides absolute quantification of DNA by partitioning the sample into thousands of individual reactions.
Multiplex PCR
Multiplex PCR is a technique that amplifies multiple DNA targets simultaneously in a single reaction using multiple primer pairs.
qPCR (Real-time PCR)
Quantitative PCR (qPCR) is a technique that amplifies and simultaneously quantifies DNA in real time using fluorescent markers.
RT-PCR
Reverse transcription PCR (RT-PCR) is a technique used to detect and amplify RNA by first converting it to complementary DNA.
PCR
PCR is a laboratory technique used to make millions of copies of a specific DNA segment through thermal cycling.
