Chromatin Immunoprecipitation
Chromatin immunoprecipitation is a method for mapping protein-DNA interactions in the genome, identifying where transcription factors and histone modifications are located.
Biochemistry CRISPR Applications Beyond Cas9
CRISPR technology has expanded far beyond genome editing with Cas9 to include base editing, prime editing, gene regulation, and diagnostic applications.
Biochemistry DNA Microarrays and Gene Expression Analysis
DNA microarrays allow simultaneous measurement of the expression levels of thousands of genes, providing a genome-wide view of cellular transcriptional activity.
Biochemistry EMSA and DNA Footprinting
EMSA and DNA footprinting are classic methods for studying protein-DNA interactions, revealing binding affinity, specificity, and the precise nucleotides contacted by proteins.
Biochemistry Flow Cytometry
Flow cytometry is a laser-based technology that rapidly measures physical and chemical properties of individual cells or particles in a fluid stream.
Biochemistry Yeast Two-Hybrid System
The yeast two-hybrid system detects protein-protein interactions in living yeast cells by reconstituting a functional transcription factor.
Biochemistry Heme Biosynthesis and Degradation
Heme is an iron-containing porphyrin that serves as the prosthetic group for hemoglobin, myoglobin, cytochromes, and other essential proteins.
Biochemistry Nucleotide Analogs as Drugs
Nucleotide analogs are a major class of therapeutic agents that interfere with nucleic acid synthesis, used extensively in cancer and antiviral therapy.
Biochemistry Purine Metabolism
Purine metabolism encompasses the de novo synthesis, salvage, and degradation of purine nucleotides, which are essential for nucleic acids, energy transfer, and signaling.
Biochemistry
