DNA Ligation & Cloning
DNA ligation and cloning is a technique used to join DNA fragments together and insert them into a vector for replication.
Biochemistry Plasmid Purification
Plasmid purification is a technique used to isolate plasmid DNA from bacterial cells for downstream applications.
Biochemistry Restriction Enzyme Digestion
Restriction enzyme digestion is a technique that uses restriction enzymes to cut DNA at specific recognition sequences.
Biochemistry The Citric Acid Cycle (Krebs Cycle)
The citric acid cycle (Krebs cycle) is a central metabolic pathway that oxidizes acetyl-CoA to produce energy and biosynthetic precursors.
Biochemistry Fatty Acid Oxidation & Synthesis
Fatty acid oxidation breaks down fatty acids for energy, while fatty acid synthesis builds them for storage and membrane formation.
Biochemistry Gluconeogenesis
Gluconeogenesis is the metabolic pathway that synthesizes glucose from non-carbohydrate precursors such as lactate and amino acids.
Biochemistry Glycolysis
Glycolysis is the metabolic pathway that converts glucose into pyruvate, producing ATP and NADH in the process.
Biochemistry Metabolic Pathways
Metabolic pathways are series of enzyme-catalyzed reactions that convert substrates into products, powering cellular processes.
Biochemistry Oxidative Phosphorylation
Oxidative phosphorylation is the process by which ATP is generated using energy derived from the electron transport chain.
Biochemistry DNA Sequencing (Sanger)
Sanger sequencing is a method for determining the nucleotide sequence of DNA using chain-terminating dideoxynucleotides.
Biochemistry Next-Generation Sequencing (NGS)
Next-generation sequencing (NGS) refers to modern high-throughput sequencing technologies that can sequence millions of DNA fragments simultaneously.
Biochemistry RNA Sequencing (RNA-Seq)
RNA sequencing (RNA-Seq) uses next-generation sequencing to analyze the transcriptome and quantify gene expression levels.
Biochemistry