Spectrophotometer Operation and Principles
Spectrophotometry measures the interaction of light with matter and is the principal quantitative technique in laboratory science.
Laboratory Fundamentals
Affinity Capture
Affinity capture is a set of biochemical techniques that purify or isolate proteins by exploiting specific binding interactions between a tagged or native protein and an immobilized capture reagent.
BiologyBiotin-Avidin Systems
Biotin-avidin systems exploit the strongest known non-covalent biological interaction to label, capture, and detect biomolecules in biochemical assays.
BiologyGST-Tag Pull-Down
GST-tag pull-down uses glutathione S-transferase fusion proteins immobilized on glutathione agarose to purify recombinant proteins or capture interacting partners.
BiologyHis-Tag / IMAC Purification
Immobilized metal affinity chromatography purifies polyhistidine-tagged recombinant proteins using immobilized metal ions and imidazole elution.
BiologyProtein A/G Purification
Protein A and Protein G are bacterial proteins that bind the Fc region of immunoglobulins, enabling rapid one-step purification of antibodies from serum or culture supernatants.
BiologyStrep-Tag Purification
Strep-tag purification uses a short peptide tag (Strep-tag II) that binds specifically to engineered streptavidin (Strep-Tactin) for gentle, one-step protein purification.
BiologyFluorescence In Situ Hybridization
Fluorescence in situ hybridization uses fluorescently labeled DNA probes to detect and localize specific DNA sequences on chromosomes and in interphase nuclei.
BiologyIn Situ Hybridization
In situ hybridization uses labeled complementary DNA or RNA probes to detect and localize specific nucleic acid sequences within intact cells, tissues, or chromosomes.
Biology
