Reporter Gene Assays
Reporter gene assays use easily measurable proteins to monitor gene expression, promoter activity, and cellular signaling pathways.
BiologyRNA Interference (siRNA, shRNA, miRNA)
RNA interference is a conserved cellular mechanism for sequence-specific gene silencing, standard to study gene function and identify therapeutic targets.
BiologyTargeted Genome Engineering: CRISPR Screening, TALENs, and Zinc Finger Nucleases
Targeted genome engineering encompasses a suite of tools, CRISPR screening, TALENs, and zinc finger nucleases, for precisely modifying or interrogating genes.
BiologyDNA and RNA Quantification Methods
Accurate quantification of nucleic acids is essential for downstream applications including sequencing, PCR, cloning, and microarray analysis.
BiologyGel Extraction and PCR Purification
Gel extraction and PCR purification are the two standard methods for purifying DNA fragments after amplification or restriction digestion.
BiologyLAMP and Isothermal Amplification
Loop-mediated isothermal amplification and related methods amplify DNA at a constant temperature, enabling rapid, point-of-care [nucleic acid detection](/guides/dna-isolation.html).
BiologyModern Cloning Methods: Gateway, Gibson Assembly, and Golden Gate
Modern cloning methods such as Gateway recombinational cloning, Gibson Assembly, and Golden Gate cloning enable seamless, modular DNA assembly without restriction enzyme digestion and ligation.
BiologyTransformation and Transfection Methods
Transformation and transfection are methods for introducing exogenous nucleic acids into bacterial and eukaryotic cells for genetic manipulation.
Biology
Analytical Weighing Techniques
Accurate weighing is the foundation of quantitative laboratory work, requiring proper balance use, environmental control, and technique.
Laboratory FundamentalsCentrifugation and Filtration Techniques
Centrifugation and filtration are fundamental separation techniques that use centrifugal force or porous media to isolate particles, cells, and molecules from liquid suspensions.
Laboratory FundamentalspH Measurement and Buffer Preparation
pH measurement and buffer preparation are essential for maintaining stable reaction conditions in virtually all biological and chemical experiments.
Laboratory FundamentalsPipetting, Dilutions, and Solution Preparation
Accurate pipetting, dilution calculations, and solution preparation are foundational skills for reproducible laboratory work.
Laboratory Fundamentals
