DNA and RNA Quantification Methods
Accurate quantification of nucleic acids is essential for downstream applications including sequencing, PCR, cloning, and microarray analysis.
BiologyGel Extraction and PCR Purification
Gel extraction and PCR purification are the two standard methods for purifying DNA fragments after amplification or restriction digestion.
BiologyLAMP and Isothermal Amplification
Loop-mediated isothermal amplification and related methods amplify DNA at a constant temperature, enabling rapid, point-of-care [nucleic acid detection](/guides/dna-isolation.html).
BiologyModern Cloning Methods: Gateway, Gibson Assembly, and Golden Gate
Modern cloning methods such as Gateway recombinational cloning, Gibson Assembly, and Golden Gate cloning enable seamless, modular DNA assembly without restriction enzyme digestion and ligation.
BiologyTransformation and Transfection Methods
Transformation and transfection are methods for introducing exogenous nucleic acids into bacterial and eukaryotic cells for genetic manipulation.
Biology
Analytical Weighing Techniques
Accurate weighing is the foundation of quantitative laboratory work, requiring proper balance use, environmental control, and technique.
Laboratory FundamentalsCentrifugation and Filtration Techniques
Centrifugation and filtration are fundamental separation techniques that use centrifugal force or porous media to isolate particles, cells, and molecules from liquid suspensions.
Laboratory FundamentalspH Measurement and Buffer Preparation
pH measurement and buffer preparation are essential for maintaining stable reaction conditions in virtually all biological and chemical experiments.
Laboratory FundamentalsPipetting, Dilutions, and Solution Preparation
Accurate pipetting, dilution calculations, and solution preparation are foundational skills for reproducible laboratory work.
Laboratory FundamentalsSpectrophotometer Operation and Principles
Spectrophotometry measures the interaction of light with matter and is the principal quantitative technique in laboratory science.
Laboratory FundamentalsAffinity Capture
Affinity capture is a set of biochemical techniques that purify or isolate proteins by exploiting specific binding interactions between a tagged or native protein and an immobilized capture reagent.
BiologyBiotin-Avidin Systems
Biotin-avidin systems exploit the strongest known non-covalent biological interaction to label, capture, and detect biomolecules in biochemical assays.
Biology