Dialysis, Ultrafiltration, and Buffer Exchange
Dialysis, ultrafiltration, and buffer exchange are essential protein handling techniques for changing buffer composition, removing small molecules, or concentrating samples.
Biology Native PAGE and Zymography
Native PAGE separates proteins in their folded, functional state, while zymography detects enzyme activity directly within an electrophoretic gel.
Biology Surface Plasmon Resonance (SPR)
Surface plasmon resonance is a label-free optical technique for measuring real-time biomolecular interactions and binding kinetics.
Biology
Immunofluorescence and Immunocytochemistry
Immunofluorescence and immunocytochemistry use labeled antibodies to visualize the location and distribution of specific proteins within fixed cells.
Biology Magnetic-Activated Cell Sorting (MACS)
Magnetic-activated cell sorting uses superparamagnetic nanoparticles and a magnetic column to isolate specific cell types from a heterogeneous suspension.
Biology
Bacterial Culture and Isolation
Bacterial culture and isolation techniques are the foundation of microbiology, enabling the growth, quantification, and purification of bacterial colonies from mixed samples.
Biology Biochemical and Automated Bacterial Identification
Bacterial identification relies on biochemical tests and automated systems to determine the genus and species of an isolate based on metabolic and enzymatic profiles.
Biology Bacterial Staining: Endospore, Capsule, Flagella, and Acid-Fast
Special staining techniques visualize bacterial structures beyond the Gram stain, including endospores, capsules, flagella, and acid-fast cell walls.
Biology Fungal Culture and Anaerobic Methods
Fungal culture and anaerobic culture methods extend microbiological analysis to organisms with specialized growth requirements beyond standard aerobic bacteria.
Biology
Plaque Assay and Viral Titration
Plaque assays and viral titration methods quantify infectious virus particles by measuring the cytopathic effect on cultured cells.
Biology
Three-Dimensional Cell Culture and Organoids
3D cell culture methods, including spheroids and organoids, provide more physiologically relevant models than traditional monolayer cultures by mimicking tissue architecture.
Biology Apoptosis and Cell Cycle Analysis
Apoptosis detection and cell cycle analysis are fundamental for understanding cell death mechanisms and the effects of drugs or genetic perturbations on cell division.
Biology