Gluconeogenesis
Gluconeogenesis is the metabolic pathway that synthesizes glucose from non-carbohydrate precursors such as lactate and amino acids.
BiochemistryGlycolysis
Glycolysis is the metabolic pathway that converts glucose into pyruvate, producing ATP and NADH in the process.
BiochemistryMetabolic Pathways
Metabolic pathways are series of enzyme-catalyzed reactions that convert substrates into products, powering cellular processes.
BiochemistryOxidative Phosphorylation
Oxidative phosphorylation is the process by which ATP is generated using energy derived from the electron transport chain.
BiochemistryDNA Sequencing (Sanger)
Sanger sequencing is a method for determining the nucleotide sequence of DNA using chain-terminating dideoxynucleotides.
BiochemistryNext-Generation Sequencing (NGS)
Next-generation sequencing (NGS) refers to modern high-throughput sequencing technologies that can sequence millions of DNA fragments simultaneously.
BiochemistryRNA Sequencing (RNA-Seq)
RNA sequencing (RNA-Seq) uses next-generation sequencing to analyze the transcriptome and quantify gene expression levels.
BiochemistryColony PCR
Colony PCR is a technique used to screen bacterial colonies for the presence of a correct DNA insert after cloning.
BiochemistryDigital PCR
Digital PCR (dPCR) is a technique that provides absolute quantification of DNA by partitioning the sample into thousands of individual reactions.
BiochemistryMultiplex PCR
Multiplex PCR is a technique that amplifies multiple DNA targets simultaneously in a single reaction using multiple primer pairs.
BiochemistryqPCR (Real-time PCR)
Quantitative PCR (qPCR) is a technique that amplifies and simultaneously quantifies DNA in real time using fluorescent markers.
BiochemistryRT-PCR
Reverse transcription PCR (RT-PCR) is a technique used to detect and amplify RNA by first converting it to complementary DNA.
Biochemistry