Cell Counting, Viability, and Proliferation Assays
Cell counting and viability assessment are essential for standardizing experimental conditions, while proliferation assays measure the rate of cell division over time.
Biology Cell Culture Fundamentals
Cell culture is the practice of growing and maintaining cells outside their native environment under controlled laboratory conditions.
Biology Cell Migration and Invasion Assays
Cell migration and invasion assays measure the ability of cells to move across surfaces or through barriers, key processes in development, immunity, and cancer metastasis.
Biology
Cryopreservation and Cell Banking
Cryopreservation enables long-term storage of viable cells, tissues, and microorganisms by cooling them to sub-zero temperatures while preventing ice crystal damage.
Biology Epigenomics: ATAC-Seq and Bisulfite Sequencing
ATAC-seq maps open chromatin regions to identify regulatory elements, while bisulfite sequencing profiles DNA methylation patterns across the genome.
Biology Reporter Gene Assays
Reporter gene assays use easily measurable proteins to monitor gene expression, promoter activity, and cellular signaling pathways.
Biology RNA Interference (siRNA, shRNA, miRNA)
RNA interference is a conserved cellular mechanism for sequence-specific gene silencing, widely used to study gene function and identify therapeutic targets.
Biology Targeted Genome Engineering: CRISPR Screening, TALENs, and Zinc Finger Nucleases
Targeted genome engineering encompasses a suite of tools — CRISPR screening, TALENs, and zinc finger nucleases — for precisely modifying or interrogating genes.
Biology DNA and RNA Quantification Methods
Accurate quantification of nucleic acids is essential for downstream applications including sequencing, PCR, cloning, and microarray analysis.
Biology Gel Extraction and PCR Purification
Gel extraction and PCR purification are the two standard methods for purifying DNA fragments after amplification or restriction digestion.
Biology LAMP and Isothermal Amplification
Loop-mediated isothermal amplification and related methods amplify DNA at a constant temperature, enabling rapid, point-of-care nucleic acid detection.
Biology Modern Cloning Methods: Gateway, Gibson Assembly, and Golden Gate
Modern cloning methods such as Gateway recombinational cloning, Gibson Assembly, and Golden Gate cloning enable seamless, modular DNA assembly without restriction enzyme digestion and ligation.
Biology