Small RNA Sequencing and Analysis
Small RNA sequencing captures miRNAs, siRNAs, and piRNAs that regulate gene expression post-transcriptionally.
BiologyTranscriptome Assembly: Reconstructing RNA Sequences
Transcriptome assembly reconstructs full-length transcript sequences from RNA-seq reads without a reference genome.
Biology
Amino Acid Synthesis
Amino acid synthesis pathways describe how organisms produce amino acids from metabolic precursors.
BiologyAmino Acids & their Properties
Amino acids are the building blocks of proteins, each with unique side chains that determine their chemical properties.
BiologyAgarose Gel Electrophoresis
Agarose gel electrophoresis is a technique used to separate DNA fragments by size using an agarose gel and electric current.
BiologyCRISPR/Cas9
CRISPR/Cas9 is a gene-editing technology that allows precise modification of DNA sequences in living organisms.
BiologyDNA Ligation & Cloning
DNA ligation and cloning is a technique used to join DNA fragments together and insert them into a vector for replication.
BiologyPlasmid Purification
Plasmid purification is a technique used to isolate plasmid DNA from bacterial cells for downstream applications.
BiologyRestriction Enzyme Digestion
Restriction enzyme digestion is a technique that uses restriction enzymes to cut DNA at specific recognition sequences.
BiologyThe Citric Acid Cycle (Krebs Cycle)
The citric acid cycle (Krebs cycle) is a central metabolic pathway that oxidizes acetyl-CoA to produce energy and biosynthetic precursors.
BiologyFatty Acid Oxidation & Synthesis
Fatty acid oxidation breaks down fatty acids for energy, while fatty acid synthesis builds them for storage and membrane formation.
BiologyGluconeogenesis
Gluconeogenesis is the metabolic pathway that synthesizes glucose from non-carbohydrate precursors such as lactate and amino acids.
Biology