Introduction to Neurohistology
Neurohistology examines the microscopic structure of the central and peripheral nervous systems, requiring specialized handling and staining techniques.
BiologyMuscle and Nerve Biopsy Processing
Muscle and nerve biopsies require specialized processing including frozen sections, enzyme histochemistry and electron microscopy for neuromuscular diagnosis.
BiologyNeurodegenerative Disease Pathology
Neurodegenerative diseases are characterized by progressive loss of neuronal populations and accumulation of abnormal protein aggregates in the brain.
BiologyStains for Myelin, Neurons and Glia
Special stains and immunohistochemistry visualize myelin, neurons and glial cells in nervous tissue for diagnosing demyelinating and degenerative diseases.
BiologyAmyloid, Pigment and Mineral Stains
Histochemical stains for amyloid, endogenous pigments and mineral deposits identify these substances in tissue sections for specific diagnosis.
BiologyCarbohydrate and Mucin Histochemistry
Carbohydrate and mucin stains detect glycogen, glycoproteins, proteoglycans and mucins in tissue sections for diagnostic histochemistry.
BiologyConnective Tissue and Muscle Stains
Connective tissue stains differentially color collagen, elastic fibers, muscle and fibrin to evaluate fibrosis, vascular disease and tissue injury.
BiologyH&E: Formulation, Chemistry and Troubleshooting
Hematoxylin and eosin is the standard tissue stain; its chemistry and formulation determine nuclear detail and cytoplasmic contrast.
BiologyStains for Microorganisms in Tissue
Histochemical stains for microorganisms enable visualization of bacteria, fungi, mycobacteria and parasites in formalin-fixed tissue sections.
BiologyAutomated Tissue Processing: Protocols, Reagents and Troubleshooting
Automated tissue processors dehydrate, clear and infiltrate fixed tissue with paraffin wax through programmed cycles of reagent exchanges.
BiologyDecalcification Techniques
Decalcification removes calcium from mineralized tissue to enable sectioning, using acids or chelating agents that must preserve morphology and antigenicity.
BiologyEmbedding and Biobanking
Embedding orients tissue in paraffin blocks for sectioning, while biobanking preserves tissue and blocks for future research and molecular analysis.
Biology