Cell Synchronization Methods
Cell synchronization arrests cultured cells at a defined phase of the cell cycle so that population-level assays reflect a homogeneous cell-cycle state.
Biology
Serum Protein Electrophoresis
Serum protein electrophoresis separates serum proteins into albumin and globulin fractions to detect monoclonal gammopathies, inflammatory states, and protein abnormalities.
Biology
Bisulfite Sequencing and DNA Methylation Analysis
Bisulfite sequencing detects 5-methylcytosine at single-base resolution by chemically converting unmethylated cytosines to uracil while leaving methylated cytosines unchanged.
BiologyTALENs and Zinc Finger Nucleases
TALENs and zinc finger nucleases (ZFNs) are programmable DNA-binding proteins that create targeted double-strand breaks for genome editing in eukaryotic cells.
BiologyApoptosis Assays
Apoptosis assays detect programmed cell death through multiple hallmarks, caspase activation, phosphatidylserine exposure, DNA fragmentation, and mitochondrial membrane potential loss.
BiologyCell Cycle Analysis
Cell cycle analysis measures the distribution of cells across G0/G1, S, and G2/M phases using DNA content staining, BrdU incorporation, or cyclin expression markers.
BiologyBacterial Transformation
Bacterial transformation introduces exogenous plasmid DNA into competent bacterial cells, enabling cloning, protein expression, and library construction.
BiologyCRISPR Screening
CRISPR screening uses pooled single-guide RNA libraries to identify genes essential for a phenotype of interest in a high-throughput, genome-wide manner.
BiologyGateway and TOPO Cloning
Gateway recombinational cloning and TOPO cloning are rapid, PCR-based methods for [transferring DNA sequences between plasmids](/guides/recombinant-dna-technology.html) without restriction enzymes or ligase.
BiologyGibson Assembly and Golden Gate Cloning
Gibson Assembly and Golden Gate cloning enable seamless, scarless assembly of multiple DNA fragments in a single reaction for synthetic biology and large construct engineering.
BiologyTransfection Methods
Transfection delivers nucleic acids into eukaryotic cells for studying gene function, protein expression, and genetic manipulation.
Biology
