Cell Culture Fundamentals
Cell culture is the practice of growing and maintaining cells outside their native environment under controlled laboratory conditions.
Cell biologyCell Migration and Invasion Assays
Cell migration and invasion assays measure the ability of cells to move across surfaces or through barriers, key processes in development, immunity, and cancer metastasis.
Cell biology
Cryopreservation and Cell Banking
Cryopreservation enables long-term storage of viable cells, tissues, and microorganisms by cooling them to sub-zero temperatures while preventing ice crystal damage.
BiochemistryEpigenomics: ATAC-Seq and Bisulfite Sequencing
ATAC-seq maps open chromatin regions to identify regulatory elements, while bisulfite sequencing profiles DNA methylation patterns across the genome.
BiochemistryReporter Gene Assays
Reporter gene assays use easily measurable proteins to monitor gene expression, promoter activity, and cellular signaling pathways.
BiochemistryRNA Interference (siRNA, shRNA, miRNA)
RNA interference is a conserved cellular mechanism for sequence-specific gene silencing, standard to study gene function and identify therapeutic targets.
BiochemistryTargeted Genome Engineering: CRISPR Screening, TALENs, and Zinc Finger Nucleases
Targeted genome engineering encompasses a suite of tools, CRISPR screening, TALENs, and zinc finger nucleases, for precisely modifying or interrogating genes.
BiochemistryDNA and RNA Quantification Methods
Accurate quantification of nucleic acids is essential for downstream applications including sequencing, PCR, cloning, and microarray analysis.
BiochemistryGel Extraction and PCR Purification
Gel extraction and PCR purification are the two standard methods for purifying DNA fragments after amplification or restriction digestion.
Biochemistry