LAMP and Isothermal Amplification
Loop-mediated isothermal amplification and related methods amplify DNA at a constant temperature, enabling rapid, point-of-care [nucleic acid detection](/guides/dna-isolation.html).
BiochemistryModern Cloning Methods: Gateway, Gibson Assembly, and Golden Gate
Modern cloning methods such as Gateway recombinational cloning, Gibson Assembly, and Golden Gate cloning enable seamless, modular DNA assembly without restriction enzyme digestion and ligation.
BiochemistryTransformation and Transfection Methods
Transformation and transfection are methods for introducing exogenous nucleic acids into bacterial and eukaryotic cells for genetic manipulation.
BiochemistryAffinity Capture
Affinity capture is a set of biochemical techniques that purify or isolate proteins by exploiting specific binding interactions between a tagged or native protein and an immobilized capture reagent.
BiochemistryBiotin-Avidin Systems
Biotin-avidin systems exploit the strongest known non-covalent biological interaction to label, capture, and detect biomolecules in biochemical assays.
BiochemistryGST-Tag Pull-Down
GST-tag pull-down uses glutathione S-transferase fusion proteins immobilized on glutathione agarose to purify recombinant proteins or capture interacting partners.
BiochemistryHis-Tag / IMAC Purification
Immobilized metal affinity chromatography purifies polyhistidine-tagged recombinant proteins using immobilized metal ions and imidazole elution.
BiochemistryProtein A/G Purification
Protein A and Protein G are bacterial proteins that bind the Fc region of immunoglobulins, enabling rapid one-step purification of antibodies from serum or culture supernatants.
BiochemistryStrep-Tag Purification
Strep-tag purification uses a short peptide tag (Strep-tag II) that binds specifically to engineered streptavidin (Strep-Tactin) for gentle, one-step protein purification.
Biochemistry