Zymography
Zymography detects protease activity in protein samples by electrophoresis through a substrate-containing polyacrylamide gel followed by renaturation and substrate digestion.
ProteinsATAC-Seq
Assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-Seq) maps genome-wide chromatin accessibility using a hyperactive Tn5 transposase.
Molecular biology techniquesBisulfite Sequencing and DNA Methylation Analysis
Bisulfite sequencing detects 5-methylcytosine at single-base resolution by chemically converting unmethylated cytosines to uracil while leaving methylated cytosines unchanged.
Molecular biology techniquesTALENs and Zinc Finger Nucleases
TALENs and zinc finger nucleases (ZFNs) are programmable DNA-binding proteins that create targeted double-strand breaks for genome editing in eukaryotic cells.
Molecular biology techniquesBacterial Transformation
Bacterial transformation introduces exogenous plasmid DNA into competent bacterial cells, enabling cloning, protein expression, and library construction.
Molecular biology techniquesCRISPR Screening
CRISPR screening uses pooled single-guide RNA libraries to identify genes essential for a phenotype of interest in a high-throughput, genome-wide manner.
Molecular biology techniquesGateway and TOPO Cloning
Gateway recombinational cloning and TOPO cloning are rapid, PCR-based methods for [transferring DNA sequences between plasmids](/guides/recombinant-dna-technology.html) without restriction enzymes or ligase.
Molecular biology techniquesGibson Assembly and Golden Gate Cloning
Gibson Assembly and Golden Gate cloning enable seamless, scarless assembly of multiple DNA fragments in a single reaction for synthetic biology and large construct engineering.
Molecular biology techniquesTransfection Methods
Transfection delivers nucleic acids into eukaryotic cells for studying gene function, protein expression, and genetic manipulation.
Molecular biology techniques