Co-Immunoprecipitation (Co-IP)
Co-immunoprecipitation is an antibody-based method for isolating a target protein along with its interacting partners from a complex biological lysate.
ProteinsDialysis, Ultrafiltration, and Buffer Exchange
Dialysis, ultrafiltration, and buffer exchange are essential protein handling techniques for changing buffer composition, removing small molecules, or concentrating samples.
ProteinsNative PAGE and Zymography
Native PAGE separates proteins in their folded, functional state, while zymography detects enzyme activity directly within an electrophoretic gel.
ProteinsSurface Plasmon Resonance (SPR)
Surface plasmon resonance is a label-free optical technique for measuring real-time biomolecular interactions and binding kinetics.
ProteinsCryopreservation and Cell Banking
Cryopreservation enables long-term storage of viable cells, tissues, and microorganisms by cooling them to sub-zero temperatures while preventing ice crystal damage.
Molecular biology techniquesEpigenomics: ATAC-Seq and Bisulfite Sequencing
ATAC-seq maps open chromatin regions to identify regulatory elements, while bisulfite sequencing profiles DNA methylation patterns across the genome.
Molecular biology techniquesReporter Gene Assays
Reporter gene assays use easily measurable proteins to monitor gene expression, promoter activity, and cellular signaling pathways.
Molecular biology techniquesRNA Interference (siRNA, shRNA, miRNA)
RNA interference is a conserved cellular mechanism for sequence-specific gene silencing, standard to study gene function and identify therapeutic targets.
Molecular biology techniquesTargeted Genome Engineering: CRISPR Screening, TALENs, and Zinc Finger Nucleases
Targeted genome engineering encompasses a suite of tools, CRISPR screening, TALENs, and zinc finger nucleases, for precisely modifying or interrogating genes.
Molecular biology techniques