DNA and RNA Quantification Methods
Accurate quantification of nucleic acids is essential for downstream applications including sequencing, PCR, cloning, and microarray analysis.
Molecular biology techniquesGel Extraction and PCR Purification
Gel extraction and PCR purification are the two standard methods for purifying DNA fragments after amplification or restriction digestion.
Molecular biology techniquesLAMP and Isothermal Amplification
Loop-mediated isothermal amplification and related methods amplify DNA at a constant temperature, enabling rapid, point-of-care [nucleic acid detection](/guides/dna-isolation.html).
Molecular biology techniquesModern Cloning Methods: Gateway, Gibson Assembly, and Golden Gate
Modern cloning methods such as Gateway recombinational cloning, Gibson Assembly, and Golden Gate cloning enable seamless, modular DNA assembly without restriction enzyme digestion and ligation.
Molecular biology techniquesTransformation and Transfection Methods
Transformation and transfection are methods for introducing exogenous nucleic acids into bacterial and eukaryotic cells for genetic manipulation.
Molecular biology techniquesAffinity Capture
Affinity capture is a set of biochemical techniques that purify or isolate proteins by exploiting specific binding interactions between a tagged or native protein and an immobilized capture reagent.
ProteinsBiotin-Avidin Systems
Biotin-avidin systems exploit the strongest known non-covalent biological interaction to label, capture, and detect biomolecules in biochemical assays.
ProteinsGST-Tag Pull-Down
GST-tag pull-down uses glutathione S-transferase fusion proteins immobilized on glutathione agarose to purify recombinant proteins or capture interacting partners.
ProteinsHis-Tag / IMAC Purification
Immobilized metal affinity chromatography purifies polyhistidine-tagged recombinant proteins using immobilized metal ions and imidazole elution.
Proteins