Serum Protein Electrophoresis
Serum protein electrophoresis separates serum proteins into albumin and globulin fractions to detect monoclonal gammopathies, inflammatory states, and protein abnormalities.
Hematology
ATAC-Seq
Assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-Seq) maps genome-wide chromatin accessibility using a hyperactive Tn5 transposase.
BiochemistryBisulfite Sequencing and DNA Methylation Analysis
Bisulfite sequencing detects 5-methylcytosine at single-base resolution by chemically converting unmethylated cytosines to uracil while leaving methylated cytosines unchanged.
BiochemistryTALENs and Zinc Finger Nucleases
TALENs and zinc finger nucleases (ZFNs) are programmable DNA-binding proteins that create targeted double-strand breaks for genome editing in eukaryotic cells.
Biochemistry
Apoptosis Assays
Apoptosis assays detect programmed cell death through multiple hallmarks, caspase activation, phosphatidylserine exposure, DNA fragmentation, and mitochondrial membrane potential loss.
Cell biologyCell Cycle Analysis
Cell cycle analysis measures the distribution of cells across G0/G1, S, and G2/M phases using DNA content staining, BrdU incorporation, or cyclin expression markers.
Cell biologyBacterial Transformation
Bacterial transformation introduces exogenous plasmid DNA into competent bacterial cells, enabling cloning, protein expression, and library construction.
BiochemistryCRISPR Screening
CRISPR screening uses pooled single-guide RNA libraries to identify genes essential for a phenotype of interest in a high-throughput, genome-wide manner.
BiochemistryGateway and TOPO Cloning
Gateway recombinational cloning and TOPO cloning are rapid, PCR-based methods for [transferring DNA sequences between plasmids](/guides/recombinant-dna-technology.html) without restriction enzymes or ligase.
Biochemistry